pcdna3 1 vector (Addgene inc)
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Addgene inc
pcdna3 1 vector
Pcdna3 1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+ha+pcdna3+1+vector/FLAG-HA-pcDNA3%2E1-+(Plasmid+%2352535)/10__1158_slash_0008___5472__can___25___2893-62-10-12
Average 95 stars, based on 115 article reviews
Pcdna3 1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+ha+pcdna3+1+vector/FLAG-HA-pcDNA3%2E1-+(Plasmid+%2352535)/10__1158_slash_0008___5472__can___25___2893-62-10-12
Average 95 stars, based on 115 article reviews
pcdna3 1 vector - by Bioz Stars,
2026-09
95/100 stars
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Amplification:Article Title: Impaired GATE16-mediated exocytosis in exocrine tissues causes Sjögren’s syndrome-like exocrinopathy Article Snippet: Plasmid construction The cDNA of the coding region of the Vamp2 ( NM_009497 ), Vamp8 ( NM_016794 ), Snap23 (NM_00117792), and Gate16 ( NM_026693 ) genes was amplified from the SMGs of male C57BL/6J mice, using the ExpandTM High Fidelity PCR System (Roche), and then subcloned into a pMD20 vector (Takara Bio, Shiga, Japan) and named pMD20-Vamp2 , pMD20-Vamp8 , pMD20-Snap23 , and pMD20-Gate16 , respectively. .. For Vamp2 , Vamp8 , or Snap23 , the fragments amplified with specific forward and common reverse primers with Phusion® High-Fidelity DNA Polymerase (New England Biolabs) were digested by EcoRI and EcoRV (New England Biolabs) and then cloned into the Article Title: FAM72A antagonizes UNG2 to promote mutagenic repair during antibody maturation Article Snippet: .. Mouse Ung2 was amplified from Ung2 cDNA (OriGene) and cloned into Clone Assay:Article Title: Impaired GATE16-mediated exocytosis in exocrine tissues causes Sjögren’s syndrome-like exocrinopathy Article Snippet: Plasmid construction The cDNA of the coding region of the Vamp2 ( NM_009497 ), Vamp8 ( NM_016794 ), Snap23 (NM_00117792), and Gate16 ( NM_026693 ) genes was amplified from the SMGs of male C57BL/6J mice, using the ExpandTM High Fidelity PCR System (Roche), and then subcloned into a pMD20 vector (Takara Bio, Shiga, Japan) and named pMD20-Vamp2 , pMD20-Vamp8 , pMD20-Snap23 , and pMD20-Gate16 , respectively. .. For Vamp2 , Vamp8 , or Snap23 , the fragments amplified with specific forward and common reverse primers with Phusion® High-Fidelity DNA Polymerase (New England Biolabs) were digested by EcoRI and EcoRV (New England Biolabs) and then cloned into the Article Title: An integrated genetic analysis of epileptogenic brain malformed lesions. Article Snippet: .. Article Title: FAM72A antagonizes UNG2 to promote mutagenic repair during antibody maturation Article Snippet: .. Mouse Ung2 was amplified from Ung2 cDNA (OriGene) and cloned into Article Title: An integrated genetic analysis of epileptogenic brain malformed lesions Article Snippet: .. Construct:Article Title: Impaired GATE16-mediated exocytosis in exocrine tissues causes Sjögren’s syndrome-like exocrinopathy Article Snippet: Plasmid construction The cDNA of the coding region of the Vamp2 ( NM_009497 ), Vamp8 ( NM_016794 ), Snap23 (NM_00117792), and Gate16 ( NM_026693 ) genes was amplified from the SMGs of male C57BL/6J mice, using the ExpandTM High Fidelity PCR System (Roche), and then subcloned into a pMD20 vector (Takara Bio, Shiga, Japan) and named pMD20-Vamp2 , pMD20-Vamp8 , pMD20-Snap23 , and pMD20-Gate16 , respectively. .. For Vamp2 , Vamp8 , or Snap23 , the fragments amplified with specific forward and common reverse primers with Phusion® High-Fidelity DNA Polymerase (New England Biolabs) were digested by EcoRI and EcoRV (New England Biolabs) and then cloned into the Article Title: FAM72A antagonizes UNG2 to promote mutagenic repair during antibody maturation Article Snippet: .. Mouse Ung2 was amplified from Ung2 cDNA (OriGene) and cloned into Article Title: Discovery and Mechanism of 16-19F, a Novel Synthetic Lethal Inhibitor of the PRMT5•MTA Complex in MTAP-Deleted Cancer Cells. Article Snippet: Protein arginine methyltransferase 5 (PRMT5), which uniquely binds to 5′-methylthioadenosine (MTA) among the PRMT family, is emerging as an attractive epigenetic target for 5′-methylthioadenosine phosphorylase (MTAP)-deleted cancer treatments.. Here, we report the discovery of a novel inhibitor 16−19F, which is a potent binder to the PRMT5•MTA, PRMT5•SAH, and PRMT5•SAM complexes and selectively inhibited MTAP-deleted cancer cell growth.. Based on transcriptome analysis, we found that kinetochore metaphase signaling and cell cycle control of the chromosomal replication pathway were downregulated after 16−19F treatment in the MDA-MB-231 TNBC cell line. Plasmid Preparation:Article Title: EGR Proteins Mediate Interferon‐Independent Anti‐HSV‐1 Responses Through Viral and Host Targets Article Snippet: .. The human Egr1 gene was inserted between the EcoRI and HindIII sites of Article Title: An integrated genetic analysis of epileptogenic brain malformed lesions. Article Snippet: .. Article Title: FAM72A antagonizes UNG2 to promote mutagenic repair during antibody maturation Article Snippet: .. Mouse Ung2 was amplified from Ung2 cDNA (OriGene) and cloned into Article Title: An integrated genetic analysis of epileptogenic brain malformed lesions Article Snippet: .. Article Title: Impaired GATE16-mediated exocytosis in exocrine tissues causes Sjögren’s syndrome-like exocrinopathy Article Snippet: The cDNA of the coding region of the Vamp2 ( NM_009497 ), Vamp8 ( NM_016794 ), Snap23 (NM_00117792), and Gate16 ( NM_026693 ) genes was amplified from the SMGs of male C57BL/6J mice, using the ExpandTM High Fidelity PCR System (Roche), and then subcloned into a pMD20 vector (Takara Bio, Shiga, Japan) and named pMD20-Vamp2 , pMD20-Vamp8 , pMD20-Snap23 , and pMD20-Gate16 , respectively. .. For Vamp2 , Vamp8 , or Snap23 , the fragments amplified with specific forward and common reverse primers with Phusion® High-Fidelity DNA Polymerase (New England Biolabs) were digested by EcoRI and EcoRV (New England Biolabs) and then cloned into the Expressing:Article Title: FAM72A antagonizes UNG2 to promote mutagenic repair during antibody maturation Article Snippet: .. Mouse Ung2 was amplified from Ung2 cDNA (OriGene) and cloned into Retroviral:Article Title: FAM72A antagonizes UNG2 to promote mutagenic repair during antibody maturation Article Snippet: .. Mouse Ung2 was amplified from Ung2 cDNA (OriGene) and cloned into Cloning:Article Title: Discovery and Mechanism of 16-19F, a Novel Synthetic Lethal Inhibitor of the PRMT5•MTA Complex in MTAP-Deleted Cancer Cells. Article Snippet: Protein arginine methyltransferase 5 (PRMT5), which uniquely binds to 5′-methylthioadenosine (MTA) among the PRMT family, is emerging as an attractive epigenetic target for 5′-methylthioadenosine phosphorylase (MTAP)-deleted cancer treatments.. Here, we report the discovery of a novel inhibitor 16−19F, which is a potent binder to the PRMT5•MTA, PRMT5•SAH, and PRMT5•SAM complexes and selectively inhibited MTAP-deleted cancer cell growth.. Based on transcriptome analysis, we found that kinetochore metaphase signaling and cell cycle control of the chromosomal replication pathway were downregulated after 16−19F treatment in the MDA-MB-231 TNBC cell line. |